elisa kit elisa kits logo

Tel:631-424-0089
Fax:631-424-0095
Email: info@immunoclone.com

Home>>Products >>  New Products

Estrogen Receptor-alpha (phospho Tyr537) Antibody

Estrogen Receptor-alpha (phospho Tyr537) Antibody Estrogen Receptor-alpha (phospho Tyr537) Antibody

Instruction Manual!

Product name: Estrogen Receptor-alpha (phospho Tyr537) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Estrogen Receptor-alpha (phospho Tyr537) Antibody

Catalog Number:IC278237

Product Profile

Product Name Estrogen Receptor-alpha (phospho Tyr537) Antibody
Antibody Type Primary Antibodies
Productdescription
							
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.1) Sehime Temel, Endocrinology, Oct 2002; 143: 3974.
More 
Immunogen
Estrogen Receptor-alpha (phospho Tyr537) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 537 (P-L-Y (p)-D-L) derived from Human Estrogen Receptor-alpha.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouse

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol ESR1
Alternative Names
ER
ESR
ESR1
ESTR
NR3A1
More 
MolecularWeight(MW)  66 kDa
Tissue Specificity The antibody detects endogenous levels of ER-alpha only when phosphorylated at tyrosine 537.

Database Links

Entrez Gene 2099
Protein Accession NP_000116.2

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Estrogen Receptor-alpha (Phospho-Tyr537) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • Application

    Western blot analysis of extracts from K562 cells (Lane 2), using Estrogen Receptor-alpha (Phospho-Tyr537) Antibody. The lane on the left is treated with antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Copyright @ USA Immuno Clone Co., Ltd(I&C) All Rights Reserved Language:Chinese