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Estrogen Receptor a (phospho Ser104) Antibody

Estrogen Receptor a (phospho Ser104) Antibody Estrogen Receptor a (phospho Ser104) Antibody

Instruction Manual!

Product name: Estrogen Receptor a (phospho Ser104) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Estrogen Receptor a (phospho Ser104) Antibody

Catalog Number:IC278231

Product Profile

Product Name Estrogen Receptor a (phospho Ser104) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.1) Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444. Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504. Kwon, Y. K. et al. (1997) J. Neurosci. 17, 8293-8299. Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.
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Immunogen
Estrogen Receptor-a (Phospho-Ser104) antibody was raised against a peptide sequence around phosphorylation site of serine 104 (S-V-S (p) -P-S) derived from Human Estrogen Receptor-α.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouse

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol ESR1
Alternative Names
ER
ESR
Era
ESRA
ESTRR
NR3A1
SHC
SHCA
ER
ESR1
ESTR
ESTRA
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MolecularWeight(MW)  66 kDa
Tissue Specificity This antibody detects endogenous level of Estrogen Receptor-α only when phosphorylated at serine104.

Database Links

Entrez Gene 2099
Protein Accession NP_000116.2

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Phospho-Ser104).

  • Application

    Western blot analysis of lysed extracts from MCF-7 cells untreated or treated with E2 and EGF using Estrogen Receptor-α (Phospho-Ser104).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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