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ATF2 (phospho Ser 490 / 498) Antibody

ATF2 (phospho Ser 490 / 498) Antibody

Instruction Manual!

Product name:	ATF2 (phospho Ser 490 / 498) Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:	IHCWB
Storage:	For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
UOM:	100ug

ATF2 (phospho Ser 490 / 498) Antibody

Catalog Number：IC271808

Product Profile

Product Name	ATF2 (phospho Ser 490 / 498) Antibody
Antibody Type	Primary Antibodies
Productdescription	The activating transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF. Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. In addition, ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered important substrates of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF expression has also been linked to the depression in humans.1) Laifenfeld, D., Karry, R., Grauer, E., Klein, E., and Ben-Shachar, D. "ATF2, a member of the CREB/ATF family of transcription factors, in chronicstress and consequent to antidepressant treatment: animal models and humanpost-mortem brains." Neuropsychopharmacology, 29 (2004) 589 - 97. More
Immunogen	ATF-2 (Ser 490/498) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the human phospho Ser490/498 of ATF2.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
TestedApplications	IHCWB
	The antibody also recognizes the phosphorylated 54k splice form of ATF2. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB, IHC and WB.: More
SpeciesReactivit	Human
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Gene Symbol	ATF2
Alternative Names	HB16 CREB2 TREB7 CREB-2 CRE-BP1 CREBP1 Activating transcription factor 2 cAMP-dependent transcription factor ATF-2 More
Molecular Weight(MW)	74
Tissue Specificity	ATF2 antibody is specific for ~74k ATF2 phosphorylated at Ser490/498.

Database Links

Entrez Gene	1386
Protein Accession	P15336

Application

Application

Western blot of human melanoma cells incubated with varying doses of the radiometric drug NCS showing phospho-specific immunolabeling of the ATF2 protein phosphorylated at Ser 490,498.

ApplicationNotes	The antibody also recognizes the phosphorylated 54k splice form of ATF2. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB, IHC and WB.: More

Additional Information

Form	Liquid
StorageInstructions	For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Storage Buffer	100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.

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