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p70 S6 Kinase Antibody

p70 S6 Kinase Antibody

Instruction Manual!

Product name:	p70 S6 Kinase Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	IFIHCWB
Storage:	Store antibody at -20˚C for up to one year.
UOM:	100ug

p70 S6 Kinase Antibody

Catalog Number：IC287212

Product Profile

Product Name	p70 S6 Kinase Antibody
Antibody Type	Primary Antibodies
Productdescription	RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .1) Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24): 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484–97 More
Immunogen	p70 S6 Kinase (Ab-424) antibody was raised against a peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
TestedApplications	IFIHCWB
	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Gene Symbol	RPS6KB1
Alternative Names	S6K PS6K S6K1 STK14A p70-S6K p70S6KA p70-alpha S6K-beta-1 p70(S6K)-alpha PKR PRKR KS6B1 P70-S6K RPS6KB1 S6K More
Molecular Weight(MW)	70, 85 kDa
Tissue Specificity	This antibody detects endogenous level of total p70 S6 Kinase protein.

Database Links

Entrez Gene	6198
Protein Accession	NP_003152.1

Application

Application

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Ab-424). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p70 S6 Kinase (Ab-424).
Application

Western blot analysis of lysed extracts from 293 cells using p70 S6 Kinase (Ab-424).

ApplicationNotes	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form	Liquid
StorageInstructions	Store antibody at -20˚C for up to one year.
Storage Buffer	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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