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p70 S6 Kinase (phospho Ser424) Antibody
Instruction Manual!
| Product name: | p70 S6 Kinase (phospho Ser424) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IFIHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
p70 S6 Kinase (phospho Ser424) Antibody
Catalog Number：IC287214
Product Profile
| Product Name | p70 S6 Kinase (phospho Ser424) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
p70 S6 Kinase (Phospho-Ser424) antibody was raised against a peptide sequence around phosphorylation site of serine 424 (P-V-S (p) -P-V) derived from Human p70 S6 Kinase. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| TestedApplications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | RPS6KB1 |
|---|---|
| Alternative Names |
S6K
More
PS6K S6K1 STK14A p70-S6K p70S6KA p70-alpha S6K-beta-1 p70(S6K)-alpha HCP PTP1C KS6B1 P70-S6K RPS6KB1 S6K |
| MolecularWeight(MW) | 70, 85 kDa |
| Tissue Specificity | This antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at serine 424. |
Database Links
| Entrez Gene | 6198 |
|---|---|
| Protein Accession | NP_003152.1 |
Application
-
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser424). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, centrosomal and nuclear staining using p70 S6 Kinase (Phospho-Ser424).
-
Application
Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase (Phospho-Ser424).
| ApplicationNotes |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
