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Raf-1 (phospho Ser642) Antibody

Raf-1 (phospho Ser642) Antibody Raf-1 (phospho Ser642) Antibody

Instruction Manual!

Product name: Raf-1 (phospho Ser642) Antibody
Source:
Purity:
Buffer Formulation:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:WB
Storage:Raf-1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
UOM:100ug

Raf-1 (phospho Ser642) Antibody

Catalog Number:IC289467

Product Profile

Product Name Raf-1 (phospho Ser642) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.1) Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469.
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Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser642 of rat Raf-1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications

WB

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, and non human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.:
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Species Reactivity

BovineChickenDogHumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol Raf1
Alternative Names
Raf
Proto-oncogene c-RAF
cRaf
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Molecular Weight(MW) 74

Database Links

Entrez Gene 24703
Protein Accession P11345

Application

ApplicationNotes
 The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, and non human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.:
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Additional Information

Form Liquid
Storage Instructions Raf-1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Storage Buffer 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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