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Mouse Raf1 Antibody
Instruction Manual!
| Product name: | Mouse Raf1 Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Applications: | FACSIHC-PWB |
| Storage: | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| UOM: | 100ug |
Mouse Raf1 Antibody
Catalog Number：IC285221
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Product Profile
| Product Name | Mouse Raf1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
This Mouse Raf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of mouse Raf1. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| TestedApplications | |
|
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | Raf1 |
|---|---|
| Alternative Names |
RAF proto-oncogene serine/threonine-protein kinase
More
Proto-oncogene c-RAF cRaf Raf-1 Raf1 Craf |
| Molecular Weight(MW) | 73 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Database Links
| Entrez Gene | 110157 |
|---|---|
| Protein Accession | Q99N57 |
Application
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Application
Mouse Raf1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
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Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
| ApplicationNotes |
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
