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Raf1 (phospho Ser259) Antibody

Raf1 (phospho Ser259) Antibody Raf1 (phospho Ser259) Antibody

Instruction Manual!

Product name: Raf1 (phospho Ser259) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Raf1 (phospho Ser259) Antibody

Catalog Number:IC289470

Product Profile

Product Name Raf1 (phospho Ser259) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.1) Dougherty M K, et al. (2005) Mol Cell. 17 (2): 215-224. Hekman M, et al. (2005) FEBS Lett. 579 (2): 464-468. Avruch J, et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183.
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Immunogen
Raf1 (Phospho-Ser259) antibody was raised against a peptide sequence around phosphorylation site of serine 259 (S-T-S (p) -T-P) derived from Human Raf1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol RAF1
Alternative Names
NS5
CRAF
Raf-1
c-Raf
BBC6
BCL2L8
C-RAF
C-Raf
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Molecular Weight(MW) 73 kDa
Tissue Specificity This antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.

Database Links

Entrez Gene 5894
Protein Accession NP_002871.1

Application

  • Application

    Western blot analysis of lysed extracts from 293 and MDA cells untreated or treated with PMA using Raf1 (Phospho-Ser259).

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1 (Phospho-Ser259).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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