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14 3 3 zeta (phospho Ser58) Antibody

14 3 3 zeta (phospho Ser58) Antibody 14 3 3 zeta (phospho Ser58) Antibody

Instruction Manual!

Product name: 14 3 3 zeta (phospho Ser58) Antibody
Source:E.coli
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

14 3 3 zeta (phospho Ser58) Antibody

Catalog Number:IC257912

Product Profile

Product Name 14 3 3 zeta (phospho Ser58) Antibody
Antibody Type Primary Antibodies
Productdescription
							
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.1) Gu YM, et al. (2006) FEBS Lett ; 580 (1): 305-310Powell DW, et al. (2003) Mol Cell Biol; 23 (15): 5376-5387Mackintosh C. (2004) Biochem J; 381 (Pt 2): 329-342.
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Immunogen
14-3-3 zeta (Phospho-Ser58) antibody was raised against a peptide sequence around phosphorylation site of serine 58 (R-S-S (p) -W-R) derived from Human 14-3-3 zeta.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol YWHAZ
MolecularWeight(MW)  28 kDa
Tissue Specificity This antibody detects endogenous level of 14-3-3 zeta only when phosphorylated at serine 58.

Database Links

Entrez Gene 7534
Protein Accession NP_001129171.1

Application

  • Application

    Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58).

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using 14-3-3 zeta (Phospho-Ser58).

ApplicationNotes 
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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