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14 3 3 zeta Antibody
Instruction Manual!
| Product name: | 14 3 3 zeta Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
14 3 3 zeta Antibody
Catalog Number：IC257976
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Product Profile
Product Name
14 3 3 zeta Antibody
Antibody Type
Primary Antibodies
Productdescription
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.1) Gu YM, et al. (2006) FEBS Lett ; 580 (1): 305-310Powell DW, et al. (2003) Mol Cell Biol; 23 (15): 5376-5387Mackintosh C. (2004) Biochem J; 381 (Pt 2): 329-342.
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Immunogen
14-3-3 zeta (Ab-58) antibody was raised against a peptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Symbol
YWHAZ
Molecular Weight(MW)
28 kDa
Tissue Specificity
This antibody detects endogenous level of total 14-3-3 zeta protein.
Database Links
Entrez Gene
7534
Protein Accession
NP_001129171.1
Application
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Application
(4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH 9.
Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Additional Information
Form
Liquid
StorageInstructions
Store antibody at -20˚C for up to one year.
Storage Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
14 3 3 zeta Antibody
Catalog Number：IC257976
Product Profile
| Product Name | 14 3 3 zeta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
14-3-3 zeta (Ab-58) antibody was raised against a peptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | YWHAZ |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Tissue Specificity | This antibody detects endogenous level of total 14-3-3 zeta protein. |
Database Links
| Entrez Gene | 7534 |
|---|---|
| Protein Accession | NP_001129171.1 |
Application
-
Application
(4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH 9.
| Application Notes |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
