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NFKB Antibody

NFKB Antibody NFKB Antibody

Instruction Manual!

Product name: NFKB Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:WB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

NFKB Antibody

Catalog Number:IC286108

Product Profile

Product Name NFKB Antibody
Antibody Type Primary Antibodies
Productdescription 
							
							
							
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.1) Beshir, A.B., et al. Cancer Lett. 299(2):137-149(2010)
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Immunogen
This NFKB(p105) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 911-937 amino acids from the C-terminal region of human NFKB(p105).

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WB

For WB starting dilution is: 1:1000:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol NFKB1
Alternative Names
Nuclear factor NF-kappa-B p105 subunit
DNA-binding factor KBF1
EBP-1
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Nuclear factor NF-kappa-B p50 subunit
NFKB1
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Molecular Weight(MW) 105 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Chicken

Database Links

Entrez Gene 4790
Protein Accession P19838

Application

  • Application

    Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the NFKB antibody detected the NFKB protein (arrow).

Application Notes
For WB starting dilution is: 1:1000:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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