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NFkB p105 / p50 (phospho Ser927) Antibody

NFkB p105 / p50 (phospho Ser927) Antibody

Instruction Manual!

Product name:	NFkB p105 / p50 (phospho Ser927) Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	IFIHCWB
Storage:	Store antibody at -20˚C for up to one year.
UOM:	100ug

NFkB p105 / p50 (phospho Ser927) Antibody

Catalog Number：IC286117

Product Profile

Product Name	NFkB p105 / p50 (phospho Ser927) Antibody
Antibody Type	Primary Antibodies
Productdescription	NF-κ-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.1) Hou S, et al. (2003) J Biol Chem. 278 (46): 45994-45998. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289. More
Immunogen	NFkB-p105/p50 (Phospho-Ser927) antibody was raised against a peptide sequence around phosphorylation site of serine 927 (C-D-S (p) -G-V) derived from Human NFκB-p105.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
TestedApplications	IFIHCWB
	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Gene Symbol	NFKB1
Alternative Names	p50 KBF1 p105 EBP-1 NF-kB1 NFKB-p50 NFkappaB NF-kappaB NFKB-p105 NF-kappa-B SHC SHCA DNA-binding factor KBF1 NF-kappa-B1 p84/NF-kappa-B1 p98 NFKB1 NFkB-p50 More
MolecularWeight(MW)	50, 120 kDa
Tissue Specificity	This antibody detects endogenous level of NFκB-p105/p50 only when phosphorylated at serine 927.

Database Links

Entrez Gene	4790
Protein Accession	NP_001158884.1

Application

Application

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p105/p50 (Phospho-Ser927). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using NFκB-p105/p50 (Phospho-Ser927).
Application

Western blot analysis of lysed extracts from HL60 cells untreated or treated with TNF-α using NFκB-p105/p50 (Phospho-Ser927).

ApplicationNotes	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form	Liquid
StorageInstructions	Store antibody at -20˚C for up to one year.
Storage Buffer	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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