elisa kit elisa kits logo

Tel:631-424-0089
Fax:631-424-0095
Email: info@immunoclone.com

Home>>Products >>  New Products

Beta-Catenin (phospho Ser33 / Ser37)Antibody

Beta-Catenin (phospho Ser33 / Ser37)Antibody Beta-Catenin (phospho Ser33 / Ser37)Antibody

Instruction Manual!

Product name: Beta-Catenin (phospho Ser33 / Ser37)Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:WB
Storage:Beta-Catenin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
UOM:100ug

Beta-Catenin (phospho Ser33 / Ser37)Antibody

Catalog Number:IC272450

Product Profile

Product Name Beta-Catenin (phospho Ser33 / Ser37)Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Beta-catenin is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway (Ding and Dale, 2002; Polakis, 2000). The role of beta-catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37 and Thr41 by glycogen synthase kinase 3beta (GSK3beta) (Liu et al., 2002). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of beta-catenin. Mutations in components of this phosphorylation regulated process that prevent beta-catenin hyperphosphorylation by GSK3beta are strongly associated with cancers (Wang et al., 2003; Polakis, 2000; Liu et al., 2002).1) Ding Y, Dale, T (2002) Wnt signal transduction: kinase cogs in a nano-machine? Trends Biochem Sci 27:327-329.
More 
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser33, 37 of human beta-catenin.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications 

WB

The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with mouse, rat and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.:
More 
Species Reactivity

HumanMouseRatXenopus

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CTNNB1
Alternative Names
CTNNB
MRD19
armadillo
CTNNB
More 
Molecular Weight(MW) 83
Sequence Similarities ORF Names: OK/SW-cl.35, PRO2286, Beta-catenin,

Database Links

Entrez Gene 1499
Protein Accession P35222

Application

  • Application

    Western blot of human embryonic kidney cell (HEK) lysate showing specific immunolabeling of the ~83k beta-catenin phosphorylated at Ser33 and Ser37 (Upper Panel) and a pan beta-catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3beta knocked down by SiRNA.

ApplicationNotes
 The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with mouse, rat and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.:
More 

Additional Information

Form Liquid
Storage Instructions Beta-Catenin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Storage Buffer 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Copyright @ USA Immuno Clone Co., Ltd(I&C) All Rights Reserved Language:Chinese