Adiponectin Antibody

Instruction Manual!

Product name:	Adiponectin Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Applications:	ELISAIHCWB
Storage:	Stable for at least 1 year after receipt when stored at -20˚C.
UOM:	100ug

Adiponectin Antibody

Catalog Number：IC269978

Product Profile

Product Name	Adiponectin Antibody
Antibody Type	Primary Antibodies
Productdescription	ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels. More
Immunogen	Recombinant human adiponectin.

Key Feature

Clonality	Monoclonal
Isotype	IgG1, k
Host Species	Mouse
TestedApplications	ELISAIHCWB
	ELISA: (direct and indirect: 1:5,000-1:10,000). Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature). Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: More
Species Reactivity	Human
Concentration	1mg/ml
Purification

Target Information

Gene Symbol	ADIPOQ
AlternativeNames	ACRP30 AdipoQ apM1 GBP28 Adipocyte Complement Related Protein of 30kDa More
Tissue Specificity	Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. Cross-reactivity with other species not tested.

Database Links

Entrez Gene	9370
Protein Accession	NP_001171271.1

Application

ApplicationNotes	ELISA: (direct and indirect: 1:5,000-1:10,000). Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature). Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: More

ApplicationNotes

ELISA: (direct and indirect: 1:5,000-1:10,000). Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature). Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.:

Form	Liquid
Storage Instructions	Stable for at least 1 year after receipt when stored at -20˚C.
Storage Buffer	Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Adiponectin Antibody

Adiponectin Antibody

Product Profile

Key Feature

Target Information

Database Links

Application

Additional Information