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4E-BP1 (phospho Thr70) Antibody

4E-BP1 (phospho Thr70) Antibody 4E-BP1 (phospho Thr70) Antibody

Instruction Manual!

Product name: 4E-BP1 (phospho Thr70) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHC
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

4E-BP1 (phospho Thr70) Antibody

Catalog Number:IC269422

Product Profile

Product Name 4E-BP1 (phospho Thr70) Antibody
Antibody Type Primary Antibodies
Productdescription
							
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.1) Pause A., Nature 371:762-767(1994).
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Immunogen
4E-BP1 (phospho Thr70) antibody was raised against a peptide sequence around phosphorylation site of threonine70 (T-K-T (p)-P-P) derived from Human 4E-BP1.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IHC

Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol EIF4EBP1
Alternative Names
4EBP1
P/OKCL.6
P/OKCL.6
PHAS-I
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MolecularWeight(MW)  12 kDa
Tissue Specificity The antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.

Database Links

Entrez Gene 1978
Protein Accession NP_004086.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Thr70) antibody (left) or the same antibody preincubated with blocking peptide (right).

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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