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4E BP1 (phospho Thr45) Antibody

4E BP1 (phospho Thr45) Antibody 4E BP1 (phospho Thr45) Antibody

Instruction Manual!

Product name: 4E BP1 (phospho Thr45) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

4E BP1 (phospho Thr45) Antibody

Catalog Number:IC258020


Product Profile

Product Name 4E BP1 (phospho Thr45) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
							
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.1) Gingras AC, et al. (1998) Genes Dev 12 (4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23): 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5): 1087-1097. Moody CA, et al. (2005) J Virol 79 (9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4): 1432-1437.
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Immunogen
4E-BP1 (Phospho-Thr45) antibody was raised against a peptide sequence around phosphorylation site of threonine 45 (S-T-T (p) -P-G) derived from Human 4E-BP1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol EIF4EBP1
MolecularWeight(MW)  18 kDa
Tissue Specificity This antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.

Database Links

Entrez Gene 1978
Protein Accession NP_004086.1

Application

  • Application

    Western blot analysis of lysed extracts from HeLa cells using 4E-BP1 (Phospho-Thr45).

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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