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HER2 (phospho Tyr1248) Antibody

HER2 (phospho Tyr1248) Antibody HER2 (phospho Tyr1248) Antibody

Instruction Manual!

Product name: HER2 (phospho Tyr1248) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IFIHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

HER2 (phospho Tyr1248) Antibody

Catalog Number:IC280814

Product Profile

Product Name HER2 (phospho Tyr1248) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.1) Yuan, Lu Qian, Ming Shi, et al. (2008) HER2-dependent MMP-7 expression is mediated by activated STAT3 Guogang Cellular Signalling, 20:1284–1291.
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Immunogen
HER2 (Phospho-Tyr1248) antibody was raised against a peptide sequence around phosphorylation site of tyrosine1248 (P-E-Y (p) -L-G) derived from Human HER2.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications

 IFIHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol ERBB2
Alternative Names
NEU
NGL
HER2
TKR1
CD340
HER-2
MLN19
HER-2/neu
AMPK1
C-erbB-2
ErbB2
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MolecularWeight(MW)  185 kDa
Tissue Specificity This antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1248.

Database Links

Entrez Gene 2064
Protein Accession NP_001005862.1

Application

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248).

  • Application

    Western blot analysis of lysed extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1248).

ApplicationNotes 
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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