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4E-BP1 Antibody

4E-BP1 Antibody

Instruction Manual!

Product name:	4E-BP1 Antibody
Source:	Mouse
Purity:	>95%
Buffer Formulation:	Ascitic fluid containing 0.03% sodium azide.
Applications:	ELISAWB
Storage:	4E-BP1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

4E-BP1 Antibody

Catalog Number：IC269427

Product Profile

Product Name	4E-BP1 Antibody
Antibody Type	Primary Antibodies
Productdescription	4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.1) Pause, A. et al. 1994.Nature. 371:762–767. More
Immunogen	Ni-NTA purified truncated recombinant 4E-BP1 expressed in E. Coli strain BL21 (DE3).

Key Feature

Clonality	Monoclonal
Isotype	IgG1
Host Species	Mouse
TestedApplications	ELISAWB
	Western Blot:1:500 - 1:1,000 IHC(P):1:500 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
Species Reactivity	Human
Concentration	1mg/ml
Purification

Target Information

Gene Symbol	EIF4EBP1
Alternative Names	Phosphorylated heat- and acid-stable protein regulated by insulin 1 4E-BP1 BP-1 4EBP1 PHAS-I More

Database Links

Entrez Gene	1978
Protein Accession	Q13541

Application

Application

Immunohistochemical analysis of paraffin - embedded human pancreas carcinoma showing cytoplasmic and membrane location using 4E - BP1 antibody with DAB staining.
Application

Western blot analysis using 4E - BP1 monoclonal antibody against truncated 4E - BP1 recombinant protein and A431 cell lysate.

PositiveControl	1) Cat. No. 1202 - A431 Cell Lysate
ApplicationNotes	We stern Blot:1:500 - 1:1,000 IHC(P):1:500 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:

Additional Information

Form	Liquid
StorageInstructions	4E-BP1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Ascitic fluid containing 0.03% sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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