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4E-BP1 (Phospho-Thr45) antibody
Instruction Manual!
| Product name: | 4E-BP1 (Phospho-Thr45) antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WBIHCELISA |
| Storage: | store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-4E-BP1 (Phospho-Thr45) polyclonal antibody
Catalog Number：IC122261
Product Profile
| ProductName | Rabbit anti-4E-BP1 (Phospho-Thr45) polyclonal antibody |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr45. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | |
|
WB:1:500~1:1000
More
IHC:1:50~1:100 ELISA:1:20000 |
|
| Species Reactivity | |
| Concentration | 1 mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | EIF4EBP1 |
|---|---|
| Gene Synonyms |
4EBP1
More
EIF4EBP1 Eukaryotic translation initiation factor 4E binding protein 1 Insulin-stimulated EIF-4E binding protein PHAS-I P/OKCL.6 PHAS-1 PHAS-I |
Database Links
| Entrez Gene | 1978 |
|---|---|
| Unigene | 411641 |
| OMIM | 602223 |
| SwissProt ID | Q13541 |
Application
-
Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.
-
Western blot
Western blot analysis of extracts from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide
| Application Notes |
WB:1:500~1:1000
More
IHC:1:50~1:100 ELISA:1:20000 |
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
