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HSP27 (phospho Ser15) Antibody
Instruction Manual!
| Product name: | HSP27 (phospho Ser15) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IFIHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
HSP27 (phospho Ser15) Antibody
Catalog Number：IC281382
Product Profile
| Product Name | HSP27 (phospho Ser15) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
HSP27 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine15 (G-P-S (p) -W-D) derived from Human HSP27. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| TestedApplications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | HSPB1 |
|---|---|
| Alternative Names |
CMT2F
More
HMN2B HSP27 HSP28 Hsp25 SRP27 HS.76067 HEL-S-102 ASPL RCC17 TUG UBXD9 UBXN9 28 kDa heat shock protein Estrogen-regulated 24 kDa protein Growth-related 25 kDa protein HSP 27 HSP25 |
| Molecular Weight(MW) | 27 kDa |
| Tissue Specificity | This antibody detects endogenous level of HSP27only when phosphorylated at serine 15. |
Database Links
| Entrez Gene | 3315 |
|---|---|
| Protein Accession | NP_001531.1 |
Application
-
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser15). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining using HSP27 (Phospho-Ser15).
-
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using HSP27 (Phospho-Ser15).
| ApplicationNotes |
W estern Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
