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Keratin 18 (phospho Ser33) Antibody

Keratin 18 (phospho Ser33) Antibody Keratin 18 (phospho Ser33) Antibody

Instruction Manual!

Product name: Keratin 18 (phospho Ser33) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Keratin 18 (phospho Ser33) Antibody

Catalog Number:IC282767

Product Profile

Product Name Keratin 18 (phospho Ser33) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.1) Ramaekers, F. C. and Bosman, F. T. (2004) J. Pathol. 204, 351-354. Chang, L. and Goldman, R. D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613. Moll, R. et al. (1982) Cell 31, 11-24.
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Immunogen
Keratin 18 (Phospho-Ser33) antibody was raised against a peptide sequence around phosphorylation site of serine 33 (A-A-S (p) -V-Y) derived from Human Keratin 18 (CK18).

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol KRT18
Alternative Names
K18
CYK18
CBL2
RNF55
CK 18
CK18
Cytokeratin endo B
K18
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MolecularWeight(MW)  46 kDa
Tissue Specificity This antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33.

Database Links

Entrez Gene 3875
Protein Accession NP_000215.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (Phospho-Ser33).

  • Application

    Western blot analysis of lysed extracts from HT29 cells untreated or treated with EGF using Keratin 18 (Phospho-Ser33).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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