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BAD (phospho Ser91/128) Antibody

BAD (phospho Ser91/128) Antibody BAD (phospho Ser91/128) Antibody

Instruction Manual!

Product name: BAD (phospho Ser91/128) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

BAD (phospho Ser91/128) Antibody

Catalog Number:IC272164

Product Profile

Product Name BAD (phospho Ser91/128) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.1) Wang H.-G., Cell 87:629-638(1996).
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Immunogen
BAD (phospho Ser91/128) antibody was raised against a peptide sequence around phosphorylation site of Serine 91 (E-P-S (p)-P-F) derived from Human BAD or Serine 128 (E-P-S (p)-P-F) derived from Mouse BAD.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol BAD
Alternative Names
BAD
BBC6
BCL2L8
Bcl- XL/Bcl-2 associated death promoter
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MolecularWeight(MW)  22 kDa
Tissue Specificity The antibody detects endogenous levels of BAD only when phosphorylated at serine 91/ serine 128.

Database Links

Entrez Gene 572
Protein Accession NP_004313.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser91/128) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • Application

    Western blot analysis of extracts from COS7 cells treated with TNF-a using BAD (Phospho-Ser91/128) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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