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BAD (phospho Ser112) Antibody

BAD (phospho Ser112) Antibody BAD (phospho Ser112) Antibody

Instruction Manual!

Product name: BAD (phospho Ser112) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

BAD (phospho Ser112) Antibody

Catalog Number:IC272161

Product Profile

Product Name BAD (phospho Ser112) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.1) Zhang B, et al. (2004) . Mol Cell Biol. 24 (14): 6205-6214. Rice PL, et al. (2003) . Cancer Res. 63 (3): 616-620. Wang XQ, et al. (2001) . J Biol Chem. 276 (48): 44504-44511.
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Immunogen
BAD (Phospho-Ser112) antibody was raised against a peptide sequence around phosphorylation site of serine 112 (H-S-S (p) -Y-P) derived from Human BAD.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol BAD
Alternative Names
BBC2
BCL2L8
ERBB
ERBB1
HER1
BBC6
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Molecular Weight(MW) 23 kDa
Tissue Specificity This antibody detects endogenous level of BAD only when phosphorylated at serine 112.

Database Links

Entrez Gene 572
Protein Accession NP_004313.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser112).

  • Application

    Western blot analysis of lysed extracts from cos7 cells untreated or treated with EGF using BAD (Phospho-Ser112).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
Storagenstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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