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Smad3 (phospho Ser213) Antibody

Smad3 (phospho Ser213) Antibody Smad3 (phospho Ser213) Antibody

Instruction Manual!

Product name: Smad3 (phospho Ser213) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:WB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Smad3 (phospho Ser213) Antibody

Catalog Number:IC291656

Product Profile

Product Name Smad3 (phospho Ser213) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.1) Zelivianski S, Cooley A, Kall R, Jeruss JS (2010) Mol Cancer Res 8, 1375-87. Alarcón C, et al. (2009)Cell 139, 757-69. Gao S, et al. (2009)Mol Cell 36, 457-68.
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Immunogen
Smad3 (phospho Ser213) antibody was raised against a peptide sequence around phosphorylation site of serine213 (P-M-S (p)-P-A) derived from Human Smad3 .

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB

Western Blot: 1:500~1:1000:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol SMAD3
Alternative Names
JV15-2
MAD-3
MADH3
Smad 3
Mothers against decapentaplegic homolog 3
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Molecular Weight(MW) 48 kDa
Tissue Specificity The antibody detects endogenous level of Smad3 only when phosphorylated at serine 213.

Database Links

Entrez Gene 4088
Protein Accession NP_001138574.1

Application

  • Application

    Western blot analysis of extracts from HT29 tissue using Smad3 (Phospho-Ser213) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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