PGR/PR Antibody

Instruction Manual!

Product name:	PGR/PR Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	IFWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC287935

Product Name	PGR/PR Antibody
Antibody Type	Primary Antibodies
Productdescription	This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform A has not been clearly determined.1) Geradts, J., et al. Cancer Invest. 28(9):969-977(2010) More
Immunogen	This PGR/PR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 816-843 amino acids from the C-terminal region of human PGR/PR.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IFWB
	For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	PGR
Alternative Names	Progesterone receptor PR Nuclear receptor subfamily 3 group C member 3 PGR NR3C3 More
Molecular Weight(MW)	99 kDa

Entrez Gene	5241
Protein Accession	P06401

Application

Western blot analysis in 293 cell line lysates (35ug/lane).
Application

Fluorescent image of U251 cell stained with PGR/PR Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PGR/PR primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7uni

Application Notes	For IF starting dilution is: 1:10~50 For WB starting dilution is: 1:1000:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.