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p38 MAPK (phospho Thr180 / Tyr182) Antibody
Instruction Manual!
| Product name: | p38 MAPK (phospho Thr180 / Tyr182) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
| Applications: | WB |
| Storage: | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| UOM: | 100ug |
p38 MAPK (phospho Thr180 / Tyr182) Antibody
Catalog Number：IC287167
Product Profile
| Product Name | p38 MAPK (phospho Thr180 / Tyr182) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
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| Immunogen |
p38 MAPK (Thr180/Tyr182) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr180/Tyr182 of p38. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| TestedApplications | |
|
Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Immunoblotting (DB) and Western Blots (WB). Immunohistochemistry (IHC): not yet determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
More
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| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | Mapk14 |
|---|---|
| Alternative Names |
RK
More
Hog p38 CRK1 CSBP Exip Mxi2 CSPB1 Csbp1 Csbp2 Prkm14 Prkm15 Sapk2A p38Hog p38alpha MAP kinase 14 |
| Molecular Weight(MW) | 39 |
| Tissue Specificity | p38 MAPK antibody is specific for the ~39k p38 protein phosphorylated at Thr180/Tyr182. |
Database Links
| Entrez Gene | 81649 |
|---|---|
| Protein Accession | P70618 |
Application
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Application
Western blot of Hela cell lysates that had been treated with +/- UV(30 min.) showing phosphospecific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.
| ApplicationNotes |
Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Immunoblotting (DB) and Western Blots (WB). Immunohistochemistry (IHC): not yet determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
More
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
