Recognises human CD221, a 155kD receptor tyrosine kinase, also known as Insulin-like growth factor I receptor (IGF-I Receptor). CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. Clone Recognises an epitope in the alpha subunits of CD221, demonstrated by Western blotting.CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin.The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221. Serotec recommend the use of MCA2344EL for this purpose. Immunohistology: This product requires protein digestion or heat with sodium citrate buffer pre-treatment of paraffin sections.
							
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