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cdc25A (phospho Ser76) Antibody

cdc25A (phospho Ser76) Antibody cdc25A (phospho Ser76) Antibody

Instruction Manual!

Product name: cdc25A (phospho Ser76) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

cdc25A (phospho Ser76) Antibody

Catalog Number:IC275046

Product Profile

Product Name cdc25A (phospho Ser76) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.1) Jin J, et al. (2003) Genes 17 (24): 3062-74. Shimuta K, et al. (2002) EMBO J 21 (14): 3694-703.
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Immunogen
cdc25A (Phospho-Ser76) antibody was raised against a peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CDC25A
Alternative Names
CDC25A2
MEK1
PRKMK1
CDC25M3
MPIP1
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Molecular Weight(MW) 70 kDa
Tissue Specificity This antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.

Database Links

Entrez Gene 993
Protein Accession NP_001780.2

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76).

  • Application

    Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF-α and CA using cdc25A (Phospho-Ser76).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
Storagenstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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