CD227/MUC1 (phospho Tyr1229) Antibody

Instruction Manual!

Product name:	CD227/MUC1 (phospho Tyr1229) Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	IFIHCWB
Storage:	Store antibody at -20˚C for up to one year.
UOM:	100ug

Catalog Number：IC274229

Product Name	CD227/MUC1 (phospho Tyr1229) Antibody
Antibody Type	Primary Antibodies
Productdescription	The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.1) Lan M.S., J. Biol. Chem. 265:15294-15299(1990). More
Immunogen	CD227/MUC1 (phospho Tyr1229) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1229 S-P-Y (p)-E-K) derived from Human CD227/MUC1.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
TestedApplications	IFIHCWB
	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	MUC1
Alternative Names	PUM EMA H23AG MUC-1 PEM More
Molecular Weight(MW)	170 kDa
Tissue Specificity	The antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.

Entrez Gene	4582
Protein Accession	NP_001018016.1

Application

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application

Immunofluorescence staining of methanol-fixed HepG2 cells using CD227/MUC1 (Phospho-Tyr1229) Antibody.
Application

Western blot analysis of extracts from HepG2 cells treated with PMA using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the antigen-specific peptide.

ApplicationNotes	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Form	Liquid
StorageInstructions	Store antibody at -20˚C for up to one year.
Storage Buffer	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.