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FKHR (phospho Ser319) Antibody

FKHR (phospho Ser319) Antibody FKHR (phospho Ser319) Antibody

Instruction Manual!

Product name: FKHR (phospho Ser319) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IFIHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

FKHR (phospho Ser319) Antibody

Catalog Number:IC278916

Product Profile

Product Name FKHR (phospho Ser319) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q141) Rena G, et al. (2002) EMBO J 21 (9): 2263-2271. Woods YL, et al. (2001) Biochem J355 (Pt 3): 597-607. Rena G, et al. (2001) Biochem J 354 (Pt 3): 605-612.
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Immunogen
FKHR (Phospho-Ser319) antibody was raised against a peptide sequence around phosphorylation site of serine 319 (T-S-S (p) -N-A) derived from Human FKHR/FOXO1A.

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications 

IFIHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity

HumanMouse

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol FOXO1
Alternative Names
FKH1
FKHR
FOXO1A
MEK2
MKK2
PRKMK2
FOXO1
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Molecular Weight(MW) 78 - 82 kDa
Tissue Specificity This antibody detects endogenous level of FKHR only when phosphorylated at serine 319.

Database Links

Entrez Gene 2308
Protein Accession NP_002006.2

Application

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser319). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using FKHR (Phospho-Ser319).

  • Application

    Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum starvation using FKHR (Phospho-Ser319).

ApplicationNotes
 Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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