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Tyrosine Hydroxylase (phospho Ser19) Antibody

Tyrosine Hydroxylase (phospho Ser19) Antibody Tyrosine Hydroxylase (phospho Ser19) Antibody

Instruction Manual!

Product name: Tyrosine Hydroxylase (phospho Ser19) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:IFIHCWB
Storage:For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:100ug

Tyrosine Hydroxylase (phospho Ser19) Antibody

Catalog Number:IC294453

Product Profile

Product Name Tyrosine Hydroxylase (phospho Ser19) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser19 in rat TH purified. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.1) Witkovsky, P., Gabriel, R., Haycock, J. W., and Meller, E., "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina," J. Chem. Neuroanat., 19 (2000) 105 - 116.
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Immunogen
TH (Ser19) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser19 of rat tyrosine hydroxylase (TH).

Key Feature

Clonality Polyclonal
Host Species Rabbit
TestedApplications 

IFIHCWB

Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:1000 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunofluorescence (IF) and Western Blot (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.:
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Species Reactivity

Rat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol Th
Alternative Names
The
Tyrosine 3-hydroxylase
TH
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MolecularWeight(MW)  60
Tissue Specificity Tyrosine Hydroxylase antibody is specific for the 60k Tyrosine Hydroxylase phosphorylated at Ser19 in PC-12 cells.

Database Links

Entrez Gene 25085
Protein Accession P04177

Application

  • Application

    Tyrosine Hydroxylase (Phospho-Ser19) Antibody

ApplicationNotes
 Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:1000 to 1:2000. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunofluorescence (IF) and Western Blot (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC, DB and WB.:
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Additional Information

Form Liquid
StorageInstructions  For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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