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Cdc25A Antibody

Cdc25A Antibody Cdc25A Antibody

Instruction Manual!

Product name: Cdc25A Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:IHC-PWB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

Cdc25A Antibody

Catalog Number:IC275049

Product Profile

Product Name Cdc25A Antibody
Antibody Type Primary Antibodies
Productdescription
							
							
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.1) Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004).
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Immunogen
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human Cdc25A.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB

For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CDC25A
Alternative Names
M-phase inducer phosphatase 1
Dual specificity phosphatase Cdc25A
CDC25A
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Molecular Weight(MW) 59 kDa

Database Links

Entrez Gene 993
Protein Accession P30304

Application

  • Application

    Western Blot at 1:2000 dilution + HCT116 whole cell lysate Lysates/proteins at 20 ug per lane.

  • Application

    Formalin-fixed and paraffin-embedded human Spleen tissue reacted with Cdc25A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Application Notes
For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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