TH Antibody

Instruction Manual!

Product name:	TH Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC293246

Product Name	TH Antibody
Antibody Type	Primary Antibodies
Productdescription	TH is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons.1) Kuhn,D.M., et.al., J. Biol. Chem. 277 (16), 14336-14342 (2002) More
Immunogen	This TH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-514 amino acids from the C-terminal region of human TH.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	TH
Alternative Names	Tyrosine 3-monooxygenase Tyrosine 3-hydroxylase TH TH TYH More
Molecular Weight(MW)	59 kDa

Entrez Gene	7054
Protein Accession	P07101

Application

Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody at 1:1000 at each lane.
Application

Western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the detected the TH protein (arrow).
Application

Formalin-fixed and paraffin-embedded human brain tissue with TH Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application

Flow cytometric analysis of K562 cells using TH Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ApplicationNotes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.