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Smad2 (phospho Ser250) Antibody

Smad2 (phospho Ser250) Antibody Smad2 (phospho Ser250) Antibody

Instruction Manual!

Product name: Smad2 (phospho Ser250) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:WB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Smad2 (phospho Ser250) Antibody

Catalog Number:IC291647

Product Profile

Product Name Smad2 (phospho Ser250) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.1) Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009)Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002)J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, Massagué J (1999)Genes Dev 13, 804-16 .
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Immunogen
Smad2 (phospho Ser250) antibody was raised against a peptide sequence around phosphorylation site of serine250 (E-L-S (p)-P-T) derived from Human Smad2 .

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB

Western Blot: 1:500~1:1000:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol SMAD2
Alternative Names
JV18-1
MADH2
Mad-related protein 2
Mothers against DPP homolog 2
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Molecular Weight(MW) 65 kDa
Tissue Specificity The antibody detects endogenous level of Smad2 only when phosphorylated at serine 250.

Database Links

Entrez Gene 4087
Protein Accession NP_001003652.1

Application

  • Application

    Western blot analysis of extracts from 293 cells treated with PMA using Phospho-Smad2 (Ser250) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000:

Additional Information

Form Liquid
StorageInstructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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