Vimentin Antibody

Instruction Manual!

Product name:	Vimentin Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIFIHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC294936

Product Name	Vimentin Antibody
Antibody Type	Primary Antibodies
Productdescription	Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.1) References for protein: More
Immunogen	This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIFIHC-PWB
	For WB starting dilution is: 1:1000 For IF starting dilution is: 1:100 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Unpurified

Gene Symbol	VIM
Alternative Names	Vimentin VIM
Molecular Weight(MW)	54 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Xenopus

Entrez Gene	7431
Protein Accession	P08670

Application

Western Blot at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 ug per lane.
Application

Fluorescent confocal image of SY5Y cells stained with Vimentin antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Antibody Vimentin primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstaine
Application

Western blot analysis of Vimentin antibody in NCI-H460 cell line lysates (35ug/lane).
Application

Vimentin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ApplicationNotes	For WB starting dilution is: 1:1000 For IF starting dilution is: 1:100 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.