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VIM antibody - N-terminal region
Instruction Manual!
| Product name: | VIM antibody - N-terminal region |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WBIHC |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-VIM polyclonal antibody - N-terminal region
Catalog Number：IC113186
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Product Profile
| ProductName | Rabbit anti-VIM polyclonal antibody - N-terminal region |
|---|---|
| Antibodyype | Primary Antibodies |
| Immunogen |
The immunogen for anti-VIM antibody: synthetic peptide directed towards the N terminal of human VIM |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | VIM |
|---|---|
| Gene Synonyms |
FLJ36605
|
| Gene Full Name | Vimentin |
| Gene Summary |
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
More
|
| Alternative Names |
FLJ36605
|
| MolecularWeight(MW) | 54kDa |
| Sequence | 466 amino acids |
Database Links
| Entrez Gene | 7431 |
|---|---|
| SwissProt ID | P08670 |
| Protein Accession | NP_003371 |
Application
-
Immunohistochemical
Human brain stem cells
Primary Antibody Dilution :
1:500
Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution :
1:1000
Color/Signal Descriptions :
VIM: Red DAPI:Blue
Gene Name :
VIM
Submitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science -
Immunofluorescence
submitted by:Carl LundinStanford UniversityThe Vimentin antibody ParaffinP050 works well for visualization of the vimentin cytoskeleton (see attached images). We used PFA-fixed COS-7 cells and an antibody dilution of 1:400.
-
Western blot
Host: Rabbit
Target Name: VIM
Sample Tissue: Human Fetal Lung
Antibody Dilution: 1.0ug/ml -
Western blot
0.2-1 ug/ml
Positive Control: Human brain
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
