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Vimentin (phospho Ser56) Antibody

Vimentin (phospho Ser56) Antibody Vimentin (phospho Ser56) Antibody

Instruction Manual!

Product name: Vimentin (phospho Ser56) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:WB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Vimentin (phospho Ser56) Antibody

Catalog Number:IC294930

Product Profile

Product Name Vimentin (phospho Ser56) Antibody
Antibody Type Primary Antibodies
Productdescription
							
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.1) Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313.
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Immunogen
Vimentin (phospho Ser56) antibody was raised against a peptide sequence around phosphorylation site of serine 56 (A-S-S (p)-P-G) derived from Human Vimentin.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB

Western Blot: 1:500~1:1000:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol VIM
Alternative Names
VIME
vimentin
MolecularWeight(MW)  54 kDa
Tissue Specificity The antibody detects endogenous level of Vimentin only when phosphorylated at serine 56.

Database Links

Entrez Gene 7431
Protein Accession NP_003371.2

Application

  • Application

    Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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