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Elk1 (phospho Ser389) Antibody

Elk1 (phospho Ser389) Antibody Elk1 (phospho Ser389) Antibody

Instruction Manual!

Product name: Elk1 (phospho Ser389) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Elk1 (phospho Ser389) Antibody

Catalog Number:IC277848

Product Profile

Product Name Elk1 (phospho Ser389) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene1) Janknecht R, et al. (1993) EMBO J. 12 (13): 5097-5104. Marais R, et al. (1993) Cell 73:381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. Hill C S, et al. (1995) Cell. 80:199-211. Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.
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Immunogen
Elk1 (Phospho-Ser389) antibody was raised against a peptide sequence around phosphorylation site of serine 389 (P-R-S (p) -P-A) derived from Human Elk-1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol ELK1
Alternative Names
SUI1
ELK1
ETS-domain protein Elk-1
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Molecular Weight(MW) 62 kDa
Tissue Specificity This antibody detects endogenous level of Elk1 only when phosphorylated at serine 389.

Database Links

Entrez Gene 2002
Protein Accession NP_001107595.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 (Phospho-Ser389).

  • Application

    Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using Elk1 (Phospho-Ser389).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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