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PAK1 (phospho Ser199) Antibody

PAK1 (phospho Ser199) Antibody PAK1 (phospho Ser199) Antibody

Instruction Manual!

Product name: PAK1 (phospho Ser199) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

PAK1 (phospho Ser199) Antibody

Catalog Number:IC287276

Product Profile

Product Name PAK1 (phospho Ser199) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.1) Brown J.L., Curr. Biol. 6:598-605(1996).
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Immunogen
PAK1 (phospho Ser199) antibody was raised against a peptide sequence around phosphorylation site of Serine 199 (T-K-S (p)-V-I) derived from Human PAK1.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol PAK1
Alternative Names
ADRB2
PAK 1
P65-PAK
P68-PAK
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MolecularWeight(MW)  61 kDa
Tissue Specificity The antibody detects endogenous levels of PAK1/2 only when phosphorylated at serine 199.

Database Links

Entrez Gene 5058
Protein Accession NP_002567.3

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using PAK1/2 (Phospho-Ser199) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • Application

    Western blot analysis of extracts from LOVO cells treated with starved using PAK1/2 (Phospho-Ser199) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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