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PAK1 (phospho Thr402) Antibody

PAK1 (phospho Thr402) Antibody

Instruction Manual!

Product name:	PAK1 (phospho Thr402) Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:	WB
Storage:	PAK1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. Storage Buffer 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
UOM:	100ug

PAK1 (phospho Thr402) Antibody

Catalog Number：IC287279

Product Profile

Product Name	PAK1 (phospho Thr402) Antibody
Antibody Type	Primary Antibodies
Productdescription	In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).1) Boda B, Alberi S, Nikonenko I, Node-Langlois R, Jourdain P, Moosmayer M, Parisi-Jourdain L, Muller D (2004) The mental retardation protein PAK-3 contributes to synapse formation and plasticity in hippocampus. J Neurosci 24:10816-10825. More
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of rat p21 activated kinase 2 (PAK-2). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
TestedApplications	WB
	The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: More
Species Reactivity	BovineDogHumanMouseRat
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Gene Symbol	Pak1
Alternative Names	Alpha-PAK PAK-1
Molecular Weight(MW)	68/70

Database Links

Entrez Gene	29431
Protein Accession	P35465

Application

Application

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabel

ApplicationNotes	The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: More

Additional Information

Form	Liquid
Storage Instructions	PAK1 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Storage Buffer	100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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