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PAK1 (phospho Thr212) Antibody
Instruction Manual!
| Product name: | PAK1 (phospho Thr212) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IFIHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
PAK1 (phospho Thr212) Antibody
Catalog Number：IC287278
Product Profile
| Product Name | PAK1 (phospho Thr212) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
PAK1 (Phospho-Thr212) antibody was raised against a peptide sequence around phosphorylation site of threonine 212 (P-V-T (p) -P-T) derived from Human PAK1. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| TestedApplications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | PAK1 |
|---|---|
| Alternative Names |
PAKalpha
More
C1orf215 LAP18 OP18 p21-activated kinase 1 PAK-1 p65-PAK Alpha-PAK |
| MolecularWeight(MW) | 68 kDa |
| Tissue Specificity | This antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212. |
Database Links
| Entrez Gene | 5058 |
|---|---|
| Protein Accession | NP_001122092.1 |
Application
-
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 (Phospho-Thr212).
-
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with forskolin using PAK1 (Phospho-Thr212).
| ApplicationNotes |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
