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ZAP70 Antibody

ZAP70 Antibody ZAP70 Antibody

Instruction Manual!

Product name: ZAP70 Antibody
Source:Mouse
Purity:>95%
Buffer Formulation:Ascitic fluid containing 0.03% sodium azide.
Applications:ELISAIHCWB
Storage:ZAP70 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

ZAP70 Antibody

Catalog Number:IC295356

Product Profile

Product Name ZAP70 Antibody
Antibody Type Primary Antibodies
Productdescription
							
ZAP70(zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Zap70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the zap-70 gene. ZAP-70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.1) Sigal Gelkop , Gerrald D. Gish, Yael Babichev J Immunol. 2005 Dec 15;175(12):8123-32.
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Immunogen
Ni-NTA purified truncated recombinant ZAP70 expressed in E. Coli strain BL21 (DE3).

Key Feature

Clonality Monoclonal
Isotype IgG1
Host Species Mouse
TestedApplications

 ELISAIHCWB

Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
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Species Reactivity

Human

Concentration 1mg/ml
Purification

Target Information

Gene Symbol ZAP70
Alternative Names
SRK
70 kDa zeta-chain associated proteinSRK
STD
TZK
STCD
ZAP-70
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Database Links

Entrez Gene 7535
Protein Accession P43403

Application

  • Application

    Western blot analysis using Dynamin1 monoclonal antibody against truncated ZAP70 recombinant protein and Jurkat cell lysate.

  • Application

    Immunohistochemical analysis of paraffin - embedded human lymph showing cytoplasmic localization using ZAP70 antibody with DAB staining.

Positive Control 1) Cat. No. 1205 - Jurkat Cell Lysate
ApplicationNotes 
Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.:
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Additional Information

Form Liquid
StorageInstructions  ZAP70 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Ascitic fluid containing 0.03% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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