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ZAP70 Antibody

ZAP70 Antibody ZAP70 Antibody

Instruction Manual!

Product name: ZAP70 Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:IHC-PWB
Storage:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

ZAP70 Antibody

Catalog Number:IC295354

Product Profile

Product Name ZAP70 Antibody
Antibody Type Primary Antibodies
Productdescription
							
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.1) Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010)
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Immunogen
This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the N-terminal region of human ZAP70.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB

For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol ZAP70
Alternative Names
Tyrosine-protein kinase ZAP-70
70 kDa zeta-chain associated protein
Syk-related tyrosine kinase
ZAP70
SRK
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Molecular Weight(MW) 70 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Mouse

Database Links

Entrez Gene 7535
Protein Accession P43403

Application

  • Application

    Western blot analysis in Jurkat cell line lysates (35ug/lane).

  • Application

    ZAP70 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:

Additional Information

Form Liquid
StorageInstructions  Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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