elisa kit elisa kits logo

Tel:631-424-0089
Fax:631-424-0095
Email: info@immunoclone.com

Home>>Products >>  New Products

AML1 (phospho Ser435) Antibody

AML1 (phospho Ser435) Antibody AML1 (phospho Ser435) Antibody

Instruction Manual!

Product name: AML1 (phospho Ser435) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:WB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

AML1 (phospho Ser435) Antibody

Catalog Number:IC271006

Product Profile

Product Name AML1 (phospho Ser435) Antibody
Antibody Type Primary Antibodies
Productdescription
							
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.1) Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
More 
Immunogen
AML1 (phospho Ser435) antibody was raised against a peptide sequence around phosphorylation site of Serine 435 ( S-N-S (p)-P-T) derived from Human AML1.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB

Western Blot: 1:500~1:1000:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol RUNX1
Alternative Names
Acute myeloid leukemia 1 protein
CBFA2
CBFA2
alpha 2 subunit
RUNX1
More 
Molecular Weight(MW) 53 kDa
Tissue Specificity The antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.

Database Links

Entrez Gene 861
Protein Accession NP_001001890.1

Application

  • Application

    Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody. The lane on the left is treated with antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Copyright @ USA Immuno Clone Co., Ltd(I&C) All Rights Reserved Language:Chinese