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AML1 (RUNX1) Antibody

AML1 (RUNX1) Antibody AML1 (RUNX1) Antibody

Instruction Manual!

Product name: AML1 (RUNX1) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:WB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

AML1 (RUNX1) Antibody

Catalog Number:IC270073

Product Profile

Product Name AML1 (RUNX1) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.1) Mao S., Frank R. C., Zhang J., Miyazaki Y., Nimer S. D. Mol. Cell. Biol. 19:3635-3644 (1999) Pelletier N., Champagne N., Stifani S., Yang X. -J. Oncogene 21:2729-2740 (2002) Cho J. -Y., Akbarali Y., Zerbini L. F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D. -E., Libermann T. A. J. Biol. Chem. 279:19512-19522 (2004)
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Immunogen
AML1 (RUNX1) antibody was raised against a peptide sequence around aa.6~10 (D-A-S-T-S) derived from Human AML1 (RUNX1).

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

WB

Western Blot: 1:500~1:1000:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol RUNX1
Alternative Names
AML1
CBFA2
EVI-1
AMLCR1
PEBP2aB
AML1-EVI-1
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Molecular Weight(MW) 55 kDa
Tissue Specificity This antibody detects endogenous level of total AML1(RUNX1) protein.

Database Links

Entrez Gene 861
Protein Accession NP_001116079.1

Application

  • Application

    Western blot analysis of extract from P19, HeLa and 293 cells using AML1 (RUNX1).

Application Notes
Western Blot: 1:500~1:1000:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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