LCK Antibody

Instruction Manual!

Product name:	LCK Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIFIHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC283299

Product Name	LCK Antibody
Antibody Type	Primary Antibodies
Productdescription	LSK, which belongs to the SRC subfamily of Tyr protein kinases, may participate in antigen-induced T-cell activation. It binds to phosphatidylinositol 3'-kinase (PI3K) from T lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. LSK is bound to the cytoplasmic domain of either CD4 or CD8. This protein is involved in leukemias by a chromosomal translocation t(1;7)(p34;q34) which involves LCK and T-cell receptor beta chain (TCRB) genes.1) Harris, R.A., et al., Proteomics 2(2):212-223 (2002). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Boncristiano, M., et al., Eur. J. Immunol. 30(9):2632-2638 (2000). Tong, L., et al., J. Biol. Chem. 273(32):20238-20242 (1998). Yamaguchi, H., et al., Nature 384(6608):484-489 (1996). More
Immunogen	This LCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human LCK.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIFIHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
Species Reactivity	HumanMouse
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	LCK
Alternative Names	Tyrosine-protein kinase Lck Leukocyte C-terminal Src kinase LSK Lymphocyte cell-specific protein-tyrosine kinase Protein YT16 Proto-oncogene Lck T cell-specific protein-tyrosine kinase p56-LCK LCK More
Molecular Weight(MW)	58 kDa

Entrez Gene	3932
Protein Accession	P06239

Application

Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Western blot analysis of lysates from mouse thymus and rat thymus tissue lysate(from left to right), using LSK Antibody (I37) at 1:1000 at each lane.
Application

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application

Confocal immunofluorescent analysis of LCK Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

ApplicationNotes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.