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Akt2 (phospho Ser474) Antibody
Instruction Manual!
| Product name: | Akt2 (phospho Ser474) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
Akt2 (phospho Ser474) Antibody
Catalog Number：IC270727
Product Profile
| Product Name | Akt2 (phospho Ser474) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
Akt2 (Phospho-Ser474) antibody was raised against a peptide sequence around phosphorylation site of serine 474 (Q-F-S (p) -Y-S) derived from Human Akt2. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | AKT2 |
|---|---|
| Alternative Names |
PKBB
More
PRKBB HIHGHH PKBBETA RAC-BETA IKKA TCF16 PKB beta Protein kinase B RAC-PK-beta |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of Akt2 only when phosphorylated at serine 474. |
Database Links
| Entrez Gene | 208 |
|---|---|
| Protein Accession | NP_001617.1 |
Application
-
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (Phospho-Ser474).
-
Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using Akt2 (Phospho-Ser474).
| Application Notes |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
