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IkB a (phospho Tyr42) Antibody

IkB a (phospho Tyr42) Antibody IkB a (phospho Tyr42) Antibody

Instruction Manual!

Product name: IkB a (phospho Tyr42) Antibody
Source:Mouse
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHC
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

IkB a (phospho Tyr42) Antibody

Catalog Number:IC281777

Product Profile

Product Name IkB a (phospho Tyr42) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.1) Beraud C, et al. (1999) Proc Natl Acad Sci U S A 96 (2): 429-434. Sundstrom S, et al. (2005) J Virol 79 (4): 2230-2239. Liu L, et al. (1998) Mol Cell Biol 18 (7): 4221-4234. Shrivastava A, et al. (1998) J Virol 72 (12): 9722-9728.
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Immunogen
IkB-a (Phospho-Tyr42) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 42 (E-E-Y (p) -E-Q) derived from Human IkappaB-α.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHC

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

MouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol NFKBIA
Alternative Names
IKBA
MAD-3
NFKBI
CDC2
CDC28A
CDKN1
P34CDC2
IKBA
NFKBIA
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MolecularWeight(MW)  39 kDa
Tissue Specificity This antibody detects endogenous levels of IkappaB-α only when phosphorylated at tyrosine 42.

Database Links

Entrez Gene 4792
Protein Accession NP_065390.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (phospho-Tyr42).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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