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IkB a Antibody

IkB a Antibody IkB a Antibody

Instruction Manual!

Product name: IkB a Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

IkB a Antibody

Catalog Number:IC281775

Product Profile

Product Name IkB a Antibody
Antibody Type Primary Antibodies
Productdescription
							
Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.1) Beraud C, et al. (1999) Proc Natl Acad Sci U S A 96 (2): 429-434. Sundstrom S, et al. (2005) J Virol 79 (4): 2230-2239. Liu L, et al. (1998) Mol Cell Biol 18 (7): 4221-4234. Shrivastava A, et al. (1998) J Virol 72 (12): 9722-9728. Franzoso G, et al. (1997) Genes Dev 11 (24): 3482-3496.
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Immunogen
IkB-a (Ab-42) antibody was raised against a peptide sequence around aa. 40~44 (E-E-Y-E-Q) derived from Human IkappaB-α.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol NFKBIA
Alternative Names
IKBA
MAD-3
NFKBI
BSP1
MADH1
MADR1
I-kappa-B-alpha
IKBA
NF-kappaB inhibitor alpha
NFKBIA
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Molecular Weight(MW) 39 kDa
Tissue Specificity This antibody detects endogenous level of total IkappaB-α protein.

Database Links

Entrez Gene 4792
Protein Accession NP_065390.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (Ab-42).

  • Application

    Western blot analysis of lysed extracts from 293 and THP1 cells using IκB-α (Ab-42).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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