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BRCA1 (phospho Ser1423) Antibody

BRCA1 (phospho Ser1423) Antibody BRCA1 (phospho Ser1423) Antibody

Instruction Manual!

Product name: BRCA1 (phospho Ser1423) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

BRCA1 (phospho Ser1423) Antibody

Catalog Number:IC272679

Product Profile

Product Name BRCA1 (phospho Ser1423) Antibody
Antibody Type Primary Antibodies
Productdescription
							
The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation1) Kim ST, et al. (2002) Genes Dev; 16 (5): 560-570Arlt MF, et al. (2004) Mol Cell Biol; 24 (15): 6701-6709Zhang J, et al. (2004) Mol Cell Biol; 24 (2): 708-718Greenberg RA, et al. (2006) Genes Dev; 20 (1): 34-46Tibbetts RS, et al. (2000) Genes Dev; 14 (23): 2989-3002
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Immunogen
BRCA1 (Phospho-Ser1423) antibody was raised against a peptide sequence around phosphorylation site of serine 1423 (H-G-S (p) -Q-P) derived from Human BRCA1.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol BRCA1
Alternative Names
IRIS
PSCP
BRCAI
BRCC1
PNCA4
RNF53
BROVCA1
PPP1R53
ERYF1
GF1
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MolecularWeight(MW)  220 kDa
Tissue Specificity This antibody detects endogenous level of BRCA1 only when phosphorylated at serine 1423.

Database Links

Entrez Gene 672
Protein Accession NP_009225.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (Phospho-Ser1423).

  • Application

    Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using BRCA1 (Phospho-Ser1423).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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